Elham Zeini, PhD Candidate

DEPARTMENT OF CHEMISTRY

CHEMISTRY 601

ANALYTICAL/ENVIRONMENTAL SECTION

SPEAKER:            Elham Zeini, PhD Candidate

DATE:                     Monday, Nov. 14, 2011

TIME:                      12:30 pm

PLACE:                  SB 324

TITLE:                    “Magneto Immunoassays for Plasmodium falciparum Histidine-Rich Protein 2 Related to Malaria based on Magnetic Nanoparticles”

ABSTRACT:           

A magneto-enzyme-linked immunosorbent assay (ELISA) procedure has been developed for the rapid and sensitive detection of Plasmodium falciparum histidine-rich protein 2 (HRP2) related to malaria [1]. The immunological reaction for the protein HRP2 was performed in a sandwich assay. On magnetic micro- and nano-particles, a commercial monoclonal antibody towards the HRP2 protein was covalently immobilized. Then a second monoclonal antibody labelled with the enzyme horseradish peroxidase (HRP) was used to provide the electrochemical or optical signal. For the electrochemical detection of the protein HRP2, the modified magnetic particles were captured by a magneto sensor made of graphite-epoxy composite (m-GEC) and this sensor was also used as the transducer. The electrochemical magneto sensor coupled with magnetic nanoparticles resulted in better sensitivity and a lower limit of detection than other methods.

[1] M. de Souza Castilho, T. Laube, H. Yamanaka, S. Alegret, and M. I. Pividori, Analytical Chemistry, 83 (2011) 5570–5577.