DR. JENNIFER A. COBB

B. Sc. (University of Utah), Ph.D. (University of Tennessee) Assistant Professor, Department of Biochemistry and Molecular Biology. 

Affiliations: Member - Southern Alberta Cancer Research Institute.

Research Interests:

One major question in cancer genetics is to what extent chromosomal instability is the beginning of tumorigenesis.  RecQ helicases have been called caretakers of the genome and it is well documented that the loss of RecQ function leads to the breakdown in the maintenance of genome integrity and an elevated predisposition to cancer. We are investigating the function of RecQ at the molecular level using Saccharomyces cerevisiae as a model system.  It is quite evident that mechanisms that preserve genome stability in yeast are indeed the same as those which go awry in many mammalian cancers. We are focusing on the relationship between Sgs1 and the MRX complex and determining if RecQ helicases preserve fragile site stability in slow replicating regions of the genome.

We are also interested to understand the mechanism of checkpoint activation during S phase. In particular how Mec1-Ddc2 (human ATR-ATRIP) is recruited to stalled replication forks to stabilize components of the replisome. It is well understood that Mec1-Ddc2 activates Rad53 and that it is recruited to both DSBs and stalled replication forks. However, much of what is thought to happen at stalled replication forks has been implied from events at DSBs.  In mammals the affinity of ATRIP for RPA suggests a model in which ATR-ATRIP is recruited to DSBs through interaction with RPA-bound single-stranded DNA (ssDNA). Currently, we are characterizing Mec1-Ddc2 localization to stalled replication forks and the signals/factors involved in its recruitment.

See Dr. Cobb’s Website

Personnel:

Carin Pihl	Lab Manager
Mireille Tittel-Elmer	PDF
Margaret Renaud-Young	Graduate Student
Denise Bustard	Graduate Student
Sareh Moradi-Fard	Graduate Student
Irene Reimer	Administrative Assistant

 

 

 

 

 

View Pub Med for Recent publications & abstracts

Recent Publications:

• Ball, L.G., Zhang, K., Cobb, J.A., Boone, C. and Xiao, W. (2009) The yeast Shu complex couples error-free post-replication repair to homologous recombination. Mol Microbiol 73, 89-102.
• Tittel-Elmer, M.V., Alabert, C., Pasero, P., and Cobb, J.A. (2009) The MRX complex stabilizes the replisome independently of the S phase checkpoint during replication stress. EMBO Journal 28,1142-56.
• Borde, V. and Cobb, J.A. (2008) Double functions for the Mre11 complex during DNA double-strand break repair and replication. Int J Biochem Cell Biol, 41, 1249-1253. 
• Cobb, J.A. and van Attikum, H. (2009) Mapping genomic targets of DNA helicases by chromatin immunoprecipitation in Saccharomyces cerevisiae. Helicases: Methods in Molecular Biology, Humana Press- Chapter 8, 113-127.
• Cobb, J.A. and Lees-Miller, S.P. (2009) DNA double strand break: Not all foci are created equal. Cell Cycle, 9, 3.
• Ball, L.G., Zhang, K., Cobb, J.A., Boone, C. and Xiao, W. (2009) The yeast Shu complex couples error-free post-replication repair to homologous recombination. Mol Microbiol 73, 89-102.
• Tittel-Elmer, M.V., Alabert, C., Pasero, P., and Cobb, J.A. (2009) The MRX complex stabilizes the replisome independently of the S phase checkpoint during replication stress. EMBO Journal 28,1142-56.

 

Contact Information:

The University of Calgary
Southern Alberta Cancer Research Institute
2A18 HRIC, 3330 Hospital Drive NW
Calgary, AB T2N 4N1 Canada
Phone: 403 220 8580
Fax: 403 210 9563
Email: jcobb [at] ucalgary [dot] ca">jcobb [at] ucalgary [dot] ca