SACRI antibody services (SAS) has extensive experience developing monoclonal antibodies.  We offer custom hybridoma development, cell culture development including cell cloning, clone screening, and cell banking to ensure production of the best monoclonal antibodies for your needs.

SAS utilizes a four stage development procedure for generating hybridomas.

Stage 1: Immunization (5 - 6 weeks)

Balb/c mice are immunized with antigen provided by the client or custom peptide produced by SAS.  Four female mice are injected between three to five times to maximize the probability of success.

Stage 2: Cell fusion

Splenocytes from immunized mice (one or two for hybridoma production and the remaining two can be used to produced polyclonal antibodies) are fused with one of several myeloma lines available to SAS.  Fusion is completed using polyethylene glycol (PEG), and colonies expanded.

Stage 3: Clone screening and selection (2 months)

Hybridoma clones are screened and selected using enzyme-linked immunosorbant assay (ELISA) to determine the optimal antibody titre producing cells.  Positive clones with the highest titre are expanded.

Stage 4: Colony storage

Final colonies are frozen and stored and banked.  SAS can provide clients with additional colonies in the future if required.

Quality control measures

Several quality control measures are available to ensure clients are supplied with only the highest quality monoclonal antibodies including:

ELISA, SDS/PAGE, isoelectric focusing, HPLC, Western Blot

Additional functional tests can be performed upon the clients' request.

Pricing

Our standard hybridoma development procedure requires 5-6 months to complete.  Exact costs will depend upon the specific requirements of each client.  Contact us for a detailed quote.

Order monoclonal antibodies from SAS

Frequently asked questions - monoclonal antibodies

Q: What is the difference between monoclonal and polyclonal antibodies?

A: Monoclonal antibodies are produced from clones of a single hybridoma cell and as a result, recognize only a single epitope on the antigen and have a defined specificity.  Polyclonal antibodies recognize independent epitopes.

Q: What is a hybridoma?

A: Hybridomas are cells generated by fusing spleenocytes and myeloma cells, which are screened to produce antibodies against the antigen used for immunization.

Q: What host animals are available?

A: SAS currently only uses mice as host animals

Q: What type of antigens can be used?

A: Clients can provide proteins and haptens (either conjugated or unconjugated peptides).  The antigen can be provided lyophilized, in solution, or in an SDS/PAGE gel.

Q: How many animals are immunized?

A: Our standard hybridoma development protocol utilizes 4 female Balb/c mice.

Q: How many clones are included?

A: In a typical hybridoma project, X clones are delivered to the client.

Q: What yield of product is delivered?

A: Although yields can never be guaranteed, on average we recover X ml of Y mg/ml for every mouse used.

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Last modified: 09/15/05 00:00:00